Innovative system for screening and purifying active ingredients


A new system combining hydrogel-modified human umbilical vein endothelial cell membrane chromatography and high-performance liquid chromatography-mass spectrometry will revolutionize the screening and purification of active ingredients in traditional Chinese medicine.

Combining Hydrogel-modified Human Umbilical Vein Endothelial Cell Membrane Chromatography with Semi-preparative High Performance Liquid Chromatography (HPLC)-Offline-High Performance Liquid Chromatography-Mass Spectrometry for Screening and Purification of Active Ingredients in Traditional Chinese Medicine An innovative system has been developed. (HPLC-MS). This groundbreaking study was led by Hui Huang of Fujian Health University in China. Separation Science Journalwhich brings great advances in the analysis and characterization of traditional Chinese medicine (1).

Traditional analytical screening and validation systems that utilize cell membrane chromatography or two-dimensional chromatography and tandem mass spectrometry face limitations in providing prepared samples containing active ingredients found in traditional Chinese herbal medicines. Therefore, these samples cannot be used directly for subsequent studies. To overcome this challenge, the research team developed a semi-preparative cell membrane chromatography column using a hydrogel-modified carrier and human umbilical vein endothelial cells. The optimization process included fine-tuning parameters such as hydrogel polymerization, cell fragmentation, and cell membrane volume, resulting in an excellent conjugation ratio between membrane protein and carrier of 15.79 mg/g.

The newly developed column underwent rigorous evaluation using multitargeted tyrosine kinase inhibitors, demonstrating excellent specificity and reproducibility. Based on this success, the researchers combined the column with a semi-preparative high performance liquid chromatography offline high performance liquid chromatography mass spectrometry system. Through this integrative approach, they successfully screened and purified his 15 active ingredients. indigo naturalis, traditional herbal medicine. Among the compounds identified, her five main ingredients stand out: L.-Lysine, Oxyresveratrol, Tryptanthrin, Isorhamnetin, Indirubin.

Furthermore, the pharmacological effects of these ingredients were validated using cell proliferation and apoptosis assays. The results revealed the potent ability of the identified compounds to inhibit proliferation and promote apoptosis in human chronic myelogenous leukemia and human promyelocytic leukemia cells.p < 0.001). This finding not only highlights the therapeutic potential of the screened ingredients, but also validates the effectiveness of the developed system in identifying bioactive ingredients with important pharmacological effects.

In conclusion, the system developed by Hui Huang and his team has made great progress in the screening and purification of active ingredients in traditional Chinese medicine, especially in traditional Chinese medicine. Combining hydrogel-modified human umbilical vein endothelial cell membrane chromatography with semi-preparative HPLC-offline HPLC-MS, this innovative approach enables the preparation of bioactive-enriched samples. This system opens new possibilities for studying the mechanism of action and therapeutic potential of traditional Chinese medicines and paves the way for future advances in natural product-based drug discovery and development.

reference

(1) Huang, H. Dai, Y. Chan, Y. Lee, Y.; Yes, H. Guo, D. Lu, Q. Cai, X. Hydrogel-modified human umbilical vein endothelial cell membrane chromatography combined with semi-preparative high-performance liquid chromatography, off-line, high-performance liquid chromatography, and mass spectrometry to screen and purify active ingredients in traditional Chinese medicine. J. Sep. Sci. 2023. DOIs: https://doi.org/10.1002/jssc.202201010



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