Hydrogels Combining Nanozymes and Probiotics for Effective Candida Vaginitis Treatment


recently scientific progress study, Scientists have developed a novel nanozyme and probiotic combination therapy for Candida vaginitis. This demonstrates efficacy in killing causative pathogens, restoring vaginal microbiota, and reducing recurrence of vaginal infections.

Research: Probiotic Nanozyme Hydrogel Modulates Vaginal Microenvironment for Candida Vaginitis Treatment. Image credit: Saiful52 / Shutterstock.com study: Probiotic nanozyme hydrogel modulates the vaginal microenvironment for the treatment of Candida vaginitis. Image credit: Saiful52 / Shutterstock.com

Background

Candida vaginitis is a fungal infection caused by: Candida albicans. The global prevalence of this vaginal infection is 75%, and approximately 5-8% of affected women experience recurrence.Vaginal microflora, especially the main beneficial bacteria Lactic acid bacteriaplays an important role in protecting vaginal epithelial cells from microbial invasion by producing lactic acid and antimicrobial substances.

Four antifungal drugs, including clotrimazole, fluconazole, miconazole, and nystatin, are widely used to treat Candida vaginitis. However, long-term use of these drugs can damage normal vaginal cells and tissues, resulting in an imbalance in the vaginal flora, an acid-base imbalance, and recurrent infections. There is a possibility.

A therapeutic formulation containing Lactic acid bacteria It is believed to be very effective in treating bacterial vaginosis through the production of hydrogen peroxide, lactic acid and bacteriocin. However, the causative agent of Candida vaginitis is more resistant to these substances, thus causing treatment failure.

In the current study, scientists developed a responsive hyaluronic acid hydrogel containing hydroxyl radical-generating nanozymes and lactate- and hydrogen peroxide-generating agents. Lactic acid bacteria For the treatment of candida vaginitis.

Mechanism of action of hydrogel

Hyaluronic acid hydrogel was prepared with the aim of destroying hyaluronic acid. Candida albicans It restores the vaginal microflora and prevents recurring infections.

Scientists conducted a series of experiments to determine the mechanism of action of the preparation. For this reason, when administered intravaginally, hyaluronic acid hydrogels are degraded by hyaluronidase produced by hyaluronic acid hydrogels. Candida albicanswhich then leads to the local release of nanozymes and lactic acid bacteria.

Upon release, Lactic acid bacteria It produces lactic acid to normalize the vaginal microenvironment and maintain an acidic pH. A large amount of hydrogen peroxide is produced. Lactic acid bacteria It was then catalyzed by nanozymes to produce more toxic hydroxyl radicals, which then died. Candida albicans.

Functional characterization of hydrogels

Functional characterization of hyaluronan hydrogels revealed that nanozymes have the ability to disrupt hyaluronan hydrogels. Candida albicans, Even with low concentrations of hydrogen peroxide.Treatment of candida vaginitis with nanozymes Lactic acid bacteria Fungal viability is reduced by 70%. however, Lactic acid bacteria alone did not affect fungal viability.

Further analysis revealed that nanozymes and Lactic acid bacteria It is highly effective in producing hydroxyl radicals and causing severe damage to fungal cell membranes. However, the combined treatment had no adverse effects. Lactic acid bacteria.

Experiments performed using clinically isolated substances Candida albicans Furthermore, it was confirmed that the combination treatment had a strong negative effect on cell viability and colony-forming ability. Candida albicans.

Regarding long-term functional integrity, the hydrogel preparation was found to successfully retain its antifungal activity even when exposed to oxygen under vacuum conditions. Therefore, oxidation does not destroy the function of nanozymes.Lactic acid bacteria Combination when stored under vacuum conditions.

of in vivo Experiments with a mouse model of Candida vaginitis show that the combination treatment efficiently kills pathogens, maintains the microbial vaginal microenvironment, and increases the richness and diversity of the vaginal microbiota, without causing significant damage to vaginal mucosal cells. It has been shown to increase resilience and reduce the risk of recurrence.

Significance of research

In current research, the combination of nanozymes that generate hydroxyl radicals and nanozymes that generate lactic acid and hydrogen peroxide Lactic acid bacteria The formulation can be used as a topical antifungal agent to treat Candida vaginitis.

Commonly used antifungal drugs cannot effectively kill Candida albicans The combination therapy evaluated in the current study appears to effectively clear the fungus, maintain the vaginal microenvironment and microbiota, and prevent relapse.

Does not destroy toxic hydroxyl radicals produced by nanozymes Lactic acid bacteriawhich is a necessary beneficial effect to improve the vaginal microenvironment and restore the microbiota.

This study not only demonstrates the benefits of combining nanozymes and probiotics over small-molecule antifungals in treating and reducing recurrence of Candida vaginitis, but also demonstrates the translational potential of nanozymes. . ”

Reference magazine:

  • Wei, G., Liu, Q., Wang, X., other. (2023). A probiotic nanozyme hydrogel modulates the vaginal microenvironment for the treatment of Candida vaginitis. Science advances. Doi: 10.1126/sciadv.adg0949



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